WebNative-PAGEからのウエスタンでバンドが出ない. 植物の培養細胞から超音波破砕で得た粗酵素抽出液より、四量体を作る酵素をNative-PAGEからのウエスタンブロッティング … Web在一个典型的native page方法中，复合物被cn-page或bn-page分离。然后可以用其它分离方法如sds-page或等电聚焦做进一步分离。随后切割凝胶，蛋白复合物每一个部分都被分开。蛋白的每个条带可以消化后做肽链指纹图谱或重新测序。这样就可以提供一个蛋白复合物中单个蛋白的重要信息。

Blue native PAGE - PubMed

WebBlue native polyacrylamide gel electrophoresis (BN-PAGE) is performed essentially as described by Schägger and von Jagow (1991), Analytical Biochemistry, 199, 223–31. First, solubilized samples are stained with a charged (Coomassie) dye. … WebBlue native PAGE (BN-PAGE) can be used for one-step isolation of protein complexes from biological membranes and total cell and tissue homogenates. It can also be used to … morphine and heart attack

Tricine–SDS-PAGE Nature Protocols

WebGamma-secretase is an intramembrane aspartyl protease complex that cleaves type I integral membrane proteins, including the amyloid beta-protein precursor and the Notch … http://www.kenkyuu2.net/cgi-biotech2012/biotechforum.cgi?mode=view;Code=2513 WebAbstract Blue native PAGE (BN-PAGE) can be used for one-step isolation of protein complexes from biological membranes and total cell and tissue homogenates. It can also be used to determine native protein masses and oligomeric states and to identify physiological protein-protein interactions. morphine and heart rate