Thr308
WebWB analysis using 29163-1-AP. Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 29163-1-AP (Phospho-AKT … WebAkt is a protein serine/threonine kinase that is involved in the regulation of diverse cellular processes. Phosphorylation of Akt at regulatory residues Thr-308 and Ser-473 leads to its full activation. The protein phosphatase 2A (PP2A) has long been known to negatively regulate Akt activity. The PP2A holoenzyme consists of the structural subunit (A), …
Thr308
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WebAug 8, 2009 · Akt (PKB) is a critical kinase in cell-survival pathways. Its activity depends on the phosphorylation of Thr308 and Ser473, by PDK1 and mTORC2, respectively. We found that Akt can be further stimulated through phosphorylation of Ser129 by another kinase, CK2. Here we show that phosphorylation of Akt at Ser129 also facilitates its association … Webhamster AKT 1/2/3 (Thr308) in cell lysates. Detection of rat AKT 1/2/3 (Thr308) is also expected. The InstantOne ELISA™ kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
WebMore>> This Anti-phospho-Akt (Thr308) Antibody is validated for use in IH(P) for the detection of phospho-Akt (Thr308). Less<< Anti-phospho-Akt (Thr308) Antibody MSDS … WebMore>> This Anti-phospho-Akt (Thr308) Antibody is validated for use in IH(P) for the detection of phospho-Akt (Thr308). Less<< Anti-phospho-Akt (Thr308) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and …
WebFeb 23, 2024 · MCF-7, T47D and HCC1500 had activating mutations in PIK3CA gene, while loss-of-activity PTEN mutations were detected in sensitive cell lines, including ZR-75-1, which showed no changes or minimal increase in the amount of p-AKT(Ser473/Thr308) and p-ERK1/2(Thr202/Tyr204) induced by everolimus compared to the resistant cell line T47D … WebAnti-phospho-Akt (Thr308) Antibody from rabbit; Synonyms: Protein kinase B, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1; find Sigma-Aldrich-071398 MSDS, related peer-reviewed papers, technical documents, similar products & …
Webp-Akt 1/2/3 (Thr308) assay The AlphaLISA® SureFire ® Ultra™ p-Akt 1/2/3 (Thr308) assay is used to measure the phosphorylation of endogenous Akt at Threonine 308 in cellular lysates. The assay is an ideal system for the screening of both modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting
WebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt. Antibodies are purified by protein A and peptide affinity chromatography. Validations. None provided. Applications. Immunoprecipitation (IP) Reactivity. Homo sapiens (Human) Mus musculus (House mouse) small home weddingWebThe functional investigation showed that circSEMA4B and SEMA4B-211aa could significantly inhibit the proliferation and migration of BC in vivo and in vitro. Of note, SEMA4B-211aa inhibited the generation of PIP3 by binding to p85, thereby inhibiting the phosphorylation of AKT (Thr308). high water astoriaWebApr 14, 2024 · Salt-inducible kinase 1 (SIK1), an AMPK-related kinase, regulates energy response-related physiopathologic processes, such as gluconeogenesis and lipid metabolism ().Recently, SIK1, not AMPK, has been increasingly identified as a major player in tumor suppressor liver kinase 1 (LKB1)-deficient NSCLC ().However, the precise roles and … high water baggy pantsWebApr 15, 2005 · Newton and colleagues revaluated this observation and noticed that okadaic acid potentiated the phosphorylation of Akt/PKB at Thr308 to a greater extent than did Ser473, suggesting that PP2A may regulate the dephosphorylation of Thr308 whereas an okadaic acid-insensitive enzyme might act on Ser473 high water bandWebProduct Description. This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in mouse cells. The antibody is … small home use security camerasWebProduct Description. CST's PathScan® Phospho-Akt (Thr308) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects phospho-Akt … high water baggy jeansWebAll lanes : Anti-AKT (phospho T308) antibody (ab38449) at 1/1000 dilution Lane 1 : HeLa cells Lane 2 : HeLa cells treated with 0.01 U/mL Insulin for 15 minutes Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution Predicted band size: 56 kDa 10% gel. Running conditions: 60v, 30min; 120v 60min small home use silent generators